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楼主  发表于: 2009-10-18   

医学SCI 论文经典句子

18 xE:p)B-]  
Jurkat cells cultured in calcium-free RPMI-1640 medium (Gibco BRL; number H~&'`h1  
22300-107) containing calcium-free 10% FBS were triggered by anti-Fas IgM. The _nF_Rp S  
treated cells were harvested at the indicated time points and incubated with Fluo-3-AM at L3S,*LnA  
a final concentration of 1 micromolar for 30 min at 37°C (Scoltock et al., 2000). The 3:r;(IaX  
labeled cells (50,000 cells per treatment) were then analyzed by exciting the cells at 488 LY7'wONx  
nm and examining the fluorescence emission of Fluo 3 at 530 nm with a FACS Scan, Xv6s,<#\  
(Becton Dickinson). A one micromolar concentration of LPA was used as a positive z`W$/tw"  
control for Ca2+ induction. The data thus obtained was analyzed with the software Win 8AK#bna~-  
MDI 2.8 and represented as contour plots.The effect of chelating intracellular calcium on SI\zW[IL  
translocation of annexin I was studied by culturing Jurkat cells in the presence of 10 q~lmOT~E  
micromolar BAPTA-AM, with or without the addition of anti-Fas IgM. Cells were .- w*&Hd7b  
harvested and fractionated as detailed above, and the S-100 fractions were assessed by -LF^u;s8&S  
immunoblotting for presence or absence of annexin I. k;]&`c^5  
Mouse bone marrow transduction and transplantation. Oo,<zS=ICk  
Retrovirus-mediated transduction of mouse bone marrow cells was done by published N. uw2Y%  
methods49. Prestimulated low-density bone marrow cells were infected with high-titer <X:7$v6T|  
retrovirus supernatant on fibronectin-coated plates. Retrovirus supernatant was generated jl@xcs]#  
in the phoenix-gp cells with a mouse stem cell virus–based retroviral vector coexpressing {min9  
EGFP and HA–RhoH as described50. EGFP+ sorted cells were transplanted by >-Jutr<I"~  
intravenous injection into the sublethally irradiated (300 rads with a 137Cs irradiator) EBJaFz'  
Rag2-/- recipient mice. At 9 weeks after transplantation, thymus, peripheral blood, bone 0nC%tCV'  
marrow, spleen and lymph nodes from each recipient mouse were collected for analysis (sZ B-  
of EGFP+ chimerism and hematopoietic lineage by flow cytometry. Expression of `6'fX[j5  
HA–RhoH and HA–RhoHF73F83 in EGFP+ sorted thymocytes of recipient mice was Q\}5q3  
confirmed by immunoblot analysis. m)&2zV/Q  
Determination of renal morphology |#"<{RS+w  
Kidney slices were postfixed in buffered 2% OsO4, dehydrated, and embedded in an ">|fB&~A  
Araldite-EM bed 812 mixture. Large sections were cut perpendicular to the renal capsule, .(D-vkz'  
containing cortex, and medulla. Thin (1 m) sections were analyzed in a blinded manner P@)z Nik[  
for morphologic alterations, as previously detailed EP*"=_  
Patient population JpN+'/  
Patients included in the study met the following criteria: (1) biopsy-proven IMN; (2) N|wI=To  
creatinine clearance 30 ml per min per 1.73 m2; and (3) persistent proteinuria >5 g per 074)(X&:x  
24 h despite treatment with an HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) .,I^)8c  
reductase inhibitor, an ACEi, and/or ARB at maximal tolerated dose for at least 4 months. <a; <|Fm.  
The Mayo Institutional Review Board and the Research Ethical Board, University Health rF'q\tJDz  
Network, University of Toronto approved the study protocol. All patients gave written B\` 4TU}kE  
informed consent. Patients who had been on treatment with prednisone, cyclosporine, or UH2fP G  
19 ,iV|^]X3$/  
mycophenolic mofetil within the last 4 months or alkylating agents within the last 6 dL!PpLR$2  
months were not included in the study. Patients with active infection, diabetes, or a MqRpG5 .  
secondary cause of MN (for example, hepatitis B, systemic lupus erythematosus (SLE), "{Lp'+wNw  
medications, malignancies) were also excluded. l!5fuB8  
Treatment .*+%-%CbP  
At enrollment, a low-sodium (<4 g day-1) and low-protein (0.8 g per kg per day of qf-0 | w  
high-quality protein) diet was recommended and patients were encouraged to maintain Dn1aaN6  
the same diet throughout the duration of the study. All patients received a similar F$X"?fj  
conservative treatment regimen that included loop diuretics to control edema, an [f 4Nq \i  
HMG-CoA reductase inhibitor, and an ACEi combined with an ARB if tolerated. j&~`H:=E  
-Blockers and non-dihydropyridine calcium channel blockers, in that order, were added JlR (U. "  
when required to control systolic blood pressures to <135 mm Hg in >75% of the P :zZ  
readings. Patients who after a minimum of 4 months of conservative therapy and x %@n$4wk7  
maximized Ang II blockade had proteinuria >5 g per 24 h received two i.v. infusions of :(ni/,~Q  
rituximab at a dose of 1000 mg on days 1 and 15. To minimize infusion reactions, +es|0;Z4yP  
patients were premedicated with acetaminophen (1000 mg) and diphenhydramine oK\{#<gCZ  
hydrochloride (50 mg) orally. In addition, methylprednisolone (100 mg, i.v.) was given M<$a OW0  
prior to the first rituximab infusion. B-cell depletion was defined as CD19+ count a*/%EP3  
<5 cells per l at any time and B-cell recovery was defined as CD19+ cell count @M=xdZNyJ  
>15 cells per l. Patients treated with rituximab, who at month 6 had proteinuria >3 g per R<]f[  
24 h and in whom CD19+ B-cell counts had increased to >15 cells per l, received a .d/e?H:  
second course of rituximab treatment following the same protocol described above. 20h+^R3{Z  
Follow-up JlaT -j  
In all patients, clinical and laboratory parameters including complete blood counts, xQ~}9Kt\  
electrolytes, serum albumin, B-cell flow cytometry for CD19+ B cells, serum A}"aH  
immunoglobulin (IgG, IgM, IgA) levels, and a lipid panel were evaluated at study entry U}hQVpP#  
and at months 1, 3, 6, 9, and 12. Creatinine clearance and protein and creatinine excretion 'qoDFR\v  
in the urine were assessed by performing two consecutive 24-h urine collections at each m6+4}=Cn  
time point. Data were considered accurate when urinary creatinine excretion was :$ qa  
consistent with a complete 24 h collection. The mean of the two measurements was G)cEUEf d  
considered for the analysis. The presence of HACAs was evaluated at baseline and at r" 4u)H>  
months 3, 6, 9, and 12. 4 s&9A/&pC  
Method / Approach / Study/ Technique MbbKo-7 F$  
A discussion is presented of a problem-solving system jc0Trs{Jf  
To improve the efficiency of the method, the following approach may be applied. 0[L)`7  
In order to an investigation was made to find the causes of the RZ +SOZs7H  
Although large collections of rules and equations have been complied, none are generally HnlCEW,^o  
accepted u"hv _ml  
20 "1I\~]]  
This approach will be explained and discussed thoroughly in the body of the report. $-4 Zi  
This can be accomplished by v#X l  
This algorithm to compute the total cost can be described step by step as follows: ^ s.necg0  
The above preliminary analysis has provided important information 5oOF|IYi  
Various methods have been proposed for selecting an optimum... ?mq<#/qb  
These concepts have been applied to 3OZu v};k  
On the basis of the concept mentioned above, 5yl[#>qt  
This can be achieved by !TwH;#U w  
In addition, tissues were stained for infiltrating lymphocytes (CD20 and CD3), and the hAi`2GP.  
amount of interstitial fibrosis was quantified by histomorphometry. Formalin-fixed, sQl`0|VH  
paraffin-embedded sections were cut onto coated glass slides. Following heat-induced (6g;FD:"6  
antigen retrieval, sections were incubated at 20°C overnight with either anti-CD20 =wcqCW,]  
primary antibody or anti-CD3 primary antibody, both at 1:1000 dilution (Dako, Canada IVY{N/ 3|  
Inc, Mississauga, Ontario, Canada). After rinsing all sections, pretreatment with 3% xPp\OuwK  
hydrogen peroxide was performed to prevent endogenous peroxidase activation. Sections Z2 3*`yR  
were incubated with a secondary rabbit anti-mouse antibody linked with avidin–biotin Geyd VT-  
complex. Sections were counterstained with hematoxylin and examined by light U#U'iPy  
microscopy. 31*0b|Z  
The HACA assay is a proprietary bridging enzyme-linked immunosorbent assay u6#FG9W7  
performed at Genentech Inc. that measures the antibody response to rituximab in human Gm1[PAj  
serum samples. c|hT\1XR,  
In all patients, clinical and laboratory parameters including complete blood counts, _$vAitUe4S  
electrolytes, serum albumin, B-cell flow cytometry for CD19+ B cells, serum {vf4l4J(  
immunoglobulin (IgG, IgM, IgA) levels, and a lipid panel were evaluated at study entry $C^tZFq  
and at months 1, 3, 6, 9, and 12. F?y4 L9|e  
This fact suggests that a new concept &:@)ro CR  
This was accomplished by taking ... ba`V`0p-(  
The preparatory stage is very time consuming process. h8zl\  
Test are performed for validity, completeness, and compatibility Kh' 7N!  
There is little hope of achieving successful ... 6jaol'{SuH  
There has been an increasing awareness of the potential of using most ..so far made have L0QF (:F5  
not taken this approach, with the exception of #[LnDU8>9  
Only a few studies can be found. =ICakh!TO  
It is a very tedious process to go through @4FG & >kQ  
It is only when .. has been completed that .. may be effected E|~)"=  
The entire interpretation process is conducted in one's head. GFX$vn-/F  
These approaches are sometimes very tedious. z7$,m#tw  
Several techniques can be used q  W"  
A polynomial parametric model can be written as [the following]/[follows]: tX'2 $}  
A xx model is constructed/formulated using xx. umJ!j&(  
A xx model represents an xx by its xx. Op>l~{{ {  
A process decision model captures the logic essential to Eb.k:8?Tn  
From the equation above, xx is equal to the summation of xx times the ... K@R * V  
21 xk\n F0z  
The validity of a xx model can be checked using Euler's formula. \ :Vm7Zg  
Given a model, one can mathematically determine whether ... or ... _7=LSf,9  
Equations for xx need to be derived and implemented in the system. kh5VuXpe  
A number of heuristic rules have been developed for j.:I{!R#  
Optimum .. techniques can be made more reliable by ... so that M9ACaf@  
An algorithm based on the characteristic ... is used to determine -Ed<Kl  
Euler's formula states the following: }T1.~E  
The completed model should agree with the formula. j.O+e|kxU  
For manufacturing purposes, a detailed and precise model of the object is necessary T@P[jtH<d  
Engineering design models are very well defined; therefore, XFmTr@\M  
To keep the domain narrow enough to be implementable, yet wide enough to be useful. W^8MsdM  
Point of View =b_/_b$q  
from an implementation standpoint, Uw5AHq).  
From the point of view of this application, 'fS&WVR?  
From this point of view, Zadeh suggested an inference rule named xxx (CRI for short). n5s2\(  
Information is the meaningful interpretation and correlation of some aggregation of data < ?{ic2j#  
in order to allow one to make decisions. kC8M2|L  
From a practical point of view, the computational aspects of an FLC require a x3tos!Y  
simplification of the fuzzy control algorithm.  Je &O  
The use of a hammer to insert screws, although partly effective, tends to distort, destroy, 4uy:sCmu  
and generally defeat the purpose of using a screw [Kusiak AI Implications for CIM W\1V`\gF  
p.129] +.pri  
Statistical analysis -POsbb>  
Data were analyzed by one-way analysis of variance comparing the three conditions 4Gor*{  
(sham operation, ischemic AKI, and bilateral nephrectomy) at each time point. If p{0NKyOvU  
significant F-statistic from analysis of variance existed, this test was followed by Dunnett &OXm^f)K  
post hoc multiple comparison procedure with sham operation as the control group. For all 3;Y 9<  
other comparisons, Student's t-test was used. A P-value of <0.05 was considered as A(}D76o_  
statistically significant. dBb &sA-A  
Values are expressed as means s.e.m. and significance was evaluated by Mann–Whitney H63?Erh>a  
U test using GraphPad Prism, version 4.0 software (GraphPad Software Inc., San Diego, 5Ei4$T  
CA, USA). tYUg%2G  
All values are expressed as means s.d. Statistical significance (defined as P<0.05) was uWDWf5@  
evaluated using analysis of variance and Bonferroni t-tests, and the two-tailed Pearson's U%V4@iz~\m  
test, where appropriate. M.u1SB0  
Data are expressed as mean s.d., median and interquartile range, or frequencies, as ##5e:<c&[  
appropriate. Variables who deviated from the normal distribution (positively skewed) 0@>   
were log-transformed (log10) before the correlation study. 1<D^+FC4b,  
Data are represented as means s.e.m. Student's t-test and multiple comparisons with t-test /C6$B)w_*{  
post hoc analysis of variance were used as indicated below, for the comparison of n= A}X4^  
22 D $&6 8  
morphological, immunohistological and functional parameters. Statistical significance p>h}k_s  
was set at P<0.05. ]=G  dAW  
The primary efficacy parameter was defined as change in urinary protein excretion from z`:tl7  
baseline (week 0) to 12 months after treatment. The 12-month changes were tested 0lLg uBW@  
against zero using the paired t-test. Secondary end points included 6-month changes in PM^Xh*~  
protein; the number with PR or CR at 6 or 12 months; and changes in glomerular Y$0K}`{  
filtration rate (GFR), serum albumin, and lipid profiles. Study sample size was based on "?S> }G\  
the desire to have 80% power to detect a drop in urinary protein of at least 2.0 g day-1. :V~ AjV  
Assuming a two-sided hypothesis test performed at a significance level of 0.05 and an s.d. 0w!:YB,}  
of urinary protein change of 2.5 g, it was determined that 15 patients were required.2 }iRRf_   
Definition of remission status is according to the criteria established by Cattran et al.30 3U4h>T@s|  
CR was defined as proteinuria <0.3 g per 24 h, PR as proteinuria 3 g per 24 h, and a cpu|tK.t  
>50% reduction in peak proteinuria and non-response as <50% reduction in peak lS |:4U.  
proteinuria. Any patient reaching a CR or PR was considered a treatment success (V<pz2\  
The statistical significance of differences for the mean values of cytokine concentration '<1Q;3Ho  
and T cell proliferation was determined with Student's t-test. Differences with a P value ZsN3 MbY  
of less than 0.05 were considered significant.  U92?e}=]  
Statistical significance was determined by Student's t-test. Data with a P value of less ZN}U^9m=  
than 0.05 were considered significant. 8V@\$4@b!#  
In vitro and in vivo experiments were analyzed by the two-tailed Student's t-test, with P n0b{Jg *  
values less than 0.05 considered statistically significant. \me-#: Gu  
The significance of differences among groups was determined by Student's t-test and j$Vtd &  
one-way analysis of variance (SigmaStat software; Jandel).  rqEP!S^  
Comparisons !m?W+ z~J  
As well, the pros and cons of these representations from a process planning point of view >x8~?)7z  
will be discussed. b|u,[jEB  
The method of using xx to implement xx described by Zadeh (1973) appeared more 1i}p?sU  
suitable \9k{h08s  
As discussed [in the previous section]/[preciously], -)[~%n#X+t  
Relation +t}<e(  
We can not invert F' directly because it defines a many-to-one mapping. T#ehJq 5  
The relationships appear very complicate /Y8{ ?  
Lifting tasks involve complex and imprecise relationship between the task variables and E[M.q;rM  
the human operator's characteristics. ,8+Jt@L  
These methods are based on the relationship between ... and ... =' &TqiIv"  
The fundamental concept of a fuzzy rating language is that we can establish a relationship )w0K2&)A  
among terms such as high, medium, and low, and then modify these relationships. :s#&nY  
This article will thus mention the latter as well as the former. Rip[  
The former two bear a close relation to a fuzzy Cartesian product. ,S-zY\XB  
Importance  G`NGt_C  
~i=5NUE  
23 !==C@cH<N  
The emphasis is on an implementation of a general approach to rule based decision J>^KQ  
making. 9On(b|mT  
Consideration / Attention 2$1D+(5;  
Careful evaluation is necessary to ensure V#\iO  
Such a formulation does not change further considerations. :.?%e{7  
Considerable attention has been paid to HfA@tZ5q|U  
Attention should be paid to an important finding of this investigation. 'Ug-64f>  
Caution should be exercised in this process to avoid ... 89[OaT_hs  
Primary consideration is given to ... components, though others can be accommodated bf$4Z: Y  
After ... has been defined by ..., a carefully analysis is carried out/performed to determine Q3aZB*$K  
A number of factors such as ...need to be taken into consideration before making the 9P#<T7  
appropriate decision. rM)-$dZ  
It should be noted that 5j# XNc)"  
It is important to point out that ... G$CI~0Se:  
These considerations have heightened interest in the possibility of providing ... 6q@VkzF  
We should stress the fundamental importance of the xx i`EG80\[Z  
Results. 9+co `t.  
Advantages / Disadvantage Hb@P Qcj  
One of the major advantages of this new measure of xx is that it can be applied to the YE1X*'4  
experimental study of uOQl;}Lk5  
One advantage of using a .. is the ease of preparing it. O"f|gc)GLz  
It has a very fast decision making process gBJM|"_A?  
All the algorithms involve mostly logical operations. NEcE -7aT  
It can be easily and without additional cost implemented in a microprocessor;based LABLT;c  
environment. T |ZJ$E0  
It can reduce the waste of designing from scratch. }XIUz|  
The advantages of using a xx to represent xx are the following: dnstm@0k  
However, xx is not without its shortcomings. 992cy2,Fb  
In most cases, the xxx shows an improvement over the existing xxx. KU)~p"0[6]  
Compared to the existing xx, the impacts of the xx are generally reduced by 5% to 9%. TW~9<c  
The "best case" results shows a savings of 6% to 9%. Qj(|uGqm3  
Most of the existing works based on xx approach can only recognize a xx . cj9<!"6  
Most of the above methods are computational expansive and limited to xx. E~'QC  
Some other advantages of xx are the following: B&4NdL/  
The problem is the limitation of this method to a limited domain of parts. :WnF>zN  
It proved limited in application because it demanded precision in system modeling that 1g bqHxWI  
was impossible in practice. aW7)}"j4  
There are advantages to be gained in the structuring of costs and benefits, the use of xx, \EOPlyf8x  
The disadvantages of this method are also disadvantages of conventional xx approaches. dEWI8Q]  
This combines the best features of both techniques 7&{[Y^R]"  
24 <jHo2U8/"s  
Hopefully, this tool can be as the reference framework of for developing a xx platform, 3ew`e"s  
and helping the administration, marketing, and knowledge management activities in [{cMEV&  
virtual communities. ( | X?  
Results tP]-u3  
An improvement on the result shown above can be made by based on the data provided G$!JJ. )d  
Recent work has identified 1" P^!N  
Time-dependent changes of pv){R;f  
Cyclosporine-induced cell death is triggered by a non-classical phosphoinositide 3-kinase MNZD-[  
and does not require ERK activation. Ph|\%P`>%  
Much of the current work on nK; c@!~pS  
Discussion of these theories is beyond the scope of this review S6bW?8`  
Based on the information contained in this lgaE2`0 [3  
The result can be categorized into nine classes v35=4>Y  
The results are illustrated by an example % C.I2J`_  
The experimental results for each xx time are reported in Table 2. N)N\iad^  
From the results obtained so far, it seem that qZ|>{^a*  
Because of the inaccuracy of the ..., a conclusion cannot be drawn as pQf5s7  
Although much effort has been made to., this reality is far from completion. %^ z## 7^  
The results indicate that the total benefits are higher than the total costs. ^-GzWT  
Their results may then serve as guidelines for lower level models, less fuzzy and more )mF; ^3  
detailed. N 56/\1R  
Conclusion "g>, X[g  
From the discussion, one may conclude that ... %an&lcoX  
The first indication that 0281"aO  
In summary, we have shown that `bx}!;{lx  
These results suggested that 7C"&f *lEi  
Our findings have shown that F2 #s^4Ii  
Our observations have provided U#=5HzE  
This study reveals following five main findings: B%Vz -t  
To our knowledge, this is the first immunohistochemical report of both PIM and HIFs in `;vJ\$-<  
the diabetic kidney. |E#+X  
As mentioned above, 3}lT"K  
Moreover, we demonstrate that, %;-] HI  
A number of studies have elaborated a body of knowledge about >^5U XQr  
There are findings to indicate that O ELh6R  
On a descriptive level, there is agreement that for 0v/}W(  
One issue that became particularly evident from our study is ltH?Ew<]  
Our data are in agreement with the findings and models presented by previous !9ytZR*  
publications, in particular those of Jones et al SZ'2/#R>  
According to our observations, N=[# "4I  
…was not evident from our data +P5\N,,7R  
25 6Yu&'[?H$  
It could therefore be concluded that Hk8pKpn3  
our data illustrate that ^;@Q3~DpP%  
All these different aspects, as listed above, lead to 6< T@\E  
In fact, it has already been speculated that 8pZGu8  
It should be furthermore mentioned in this context that )W1tBi  
To identify and verify the essential factors triggering developmental renin expression, it pDloew  
is of interest now to study the development of intrarenal renin expression in mice with Je1'0h9d  
defined gene mutations, using the results of this study as a reference system. #D2.RN  
In conclusion, using several approaches, we demonstrated that ,VdNP  
But we have not provided formal proof yet that Q6lC:cB<  
In summary, our results suggest that 7)Y0D@wg  
In conclusion, we identify M8p6f)l3  
A better understanding of such regulatory systems may yield novel therapeutic )GC9%mF;  
approaches to glomerular diseases. ys~oJb~  
Preliminary results of short-term studies (2 weeks) indicate that Qf:#{~/  
However, we believe that nQ5N\RAZ  
However, despite our extensive measurements of these parameters, we found no Y$\c_#/]  
relationship between response and initial proteinuria, time to B-cell recovery, the a58]#L~  
development of antibodies to rituximab, B-cell count in the kidney tissue, nor the degree e_v_y$  
of tubular interstitial damage present. A clear conclusion for efficacy, however, cannot be XQhbH^  
obtained from an uncontrolled study, and definitive claims of efficacy should be reserved KW 78J~u+  
for rigorous, prospective, controlled, and randomized trials with the use of rituximab and lv=rL   
that will also look for factors that may determine its efficacy in IMN. |K,[[D<R  
It has become increasingly clear that L7rH=gZ&!]  
Form the above discussion, the conclusion can be reached that Mr6q7  
The conclusions drawn are also valid ^|j @' @L  
In conclusion to this, it becomes obvious that the problem of xx lies not only in... IL&;2%  
We have attempted to introduce some concepts associated with a theory of | ycN)zuE  
Considerable more work, hopefully, will be done in this area ?Zv>4+Y'  
Employing the compositional rule of inference, the assessment of the xx compatibility in nSR<(-j !  
achieving prescribed xx projectiles in any level of the hierarchy is made possible. r' E|6_0  
This paper has presented a theoretical and experimental study of the xx process and xx V|`w/P9g4  
concept. 3U!#rz"  
The experimental research results will hopefully serve as useful feedback information for 4E 0 Y=  
improvements for xx work. 4E 32DG*  
The scope of this contribution was to introduce a xx method. sOb=+u$$9  
This has a tremendous impact on our understanding of W%_Cda5,  
Significant progress has been made in advancing RNAi therapeutics in a remarkably D wtvtglqV  
short period of time ^w6~?'}  
To date, little is known about… m&{%6  
Irrespective of the mechanisms and the molecules –which are still largely unknown – m!LJK`gA  
involved in MSC functions, current data suggest that *DS>#x@3*i  
To better understand ay`A Gr  
26 _<m yM2z  
Future Research (常用于conclustion 的结尾) wG}R h,  
Thus, first extension of the approach could be, Q)L6+gW^  
Further studies are needed to determine the importance of D\-D ~G]x  
Some improvements to the scheduling aspect of the model may be brought through DC+l3N  
additional levels in the hierarchy for more detailed representation of the scheduling  7I;A5f  
activity. >(BAIjF E\  
It also unveiled new aspects of the role played by thyroid hormone in response to injury 0*F<tg,+]  
and tissue repair. .F^372hH3  
In the near future, the ongoing clinical trials with siRNAs for macular degeneration and Qoom[@$  
RSV may reveal the exciting potential of RNAi therapeutics as the next major class of (y!V0iy]  
drug molecules. AoFxho  
In the next few years it should become clear whether bW?cb5C  
It should be an exciting time for researchers seeking to harness this powerful endogenous zya2 O?s  
pathway to treat human disease. %pBc]n@_  
Acknowledgement \?~cJMN  
The authors are grateful to the insightful work provided by Mr. John W. Harney and all mT&?DZ9<  
of the fellows and students that contributed with some of the studies discussed in this =kDh:&u%  
article ck\TTNA  
I would like to express my gratitude to all those who gave me the possibility to complete .Hm1ispq  
this thesis. D V=xqC6}  
I want to thank the Department of Transport of the former Interim Government of FfpP<( 4  
Namibia for giving me permission to commence this thesis in the first instance, to do the @G?R (  
necessary research work and to use departmental data aK5O 0`  
This work was supported by NIH grants AI058695 and AI056900. We thank members of ,}a'h4C  
the laboratory and our collaborators for many useful suggestions. cg).b?g  
We thank J. Maraganore and B. Greene for critical reading of the manuscript, M. c$b~? Mx  
Duckman for graphics assistance and A. Capobianco for word processing assistance. u )k Q*&  
Author contributions Jm[_X  
All authors were involved in experimental planning and data analysis and contributed to PS'SIX  
manuscript preparation. h)pYV>!d  
Tables and Figures >bbvQb +j  
Figure 7-1 sketches these relationships. Yx':~  
The graphical representation of these functions is shown in Figure 1. 82KWe=  
The xx may be depicted as in Figure 1. -s&7zqW  
Figure x shows the schematic diagram of the gtRVXgI  
Figure 1 though 2 provide a ... that h%8C_m A  
the architecture of this expert system for .... is illustrated in Figure 2. . kp3<.  
Figure 2 gives the outline of an ... system {VT**o  
Table shows the @YHt[>*S  
27 5 Mz6/&`  
Time-dependent changes of T07 AH  
as shown in Table 1 and 2 h )h%y )1  
This concept is illustrated in Figure 2 EFz Pt?l  
At the top of Table xx are shown two blocks of data. rbc7CPq_^  
Each table or matrix has constructs xx through xx as row;headings, bA\(oD+:  
xx through xx as column;headings. `*y%[J,I#  
Time course of calculated renin immunoreactive tissue volumes during development of *X8Pa ;x  
the mouse kidney. Data are single values per time point. They were derived from the 349BQ5ND  
kidneys shown in Figure 1. ', P_a,\  
Yellow color indicates R#4f_9e<Z  
PCT3 cells were pretreated with 50 M PD98059 or 10 M U0126 for 30 min and then Q& S 7_  
cells were stimulated with 25 g ml-1 CsA for 6 h. ERK and PKB activation were 'RZ0,SK'  
analyzed by western blot using phospho-specific antibodies. lY[\eQ 1:  
ERK and PKB activation were measured by western blot using phospho-specific \]0+J  
antibodies N*SUA4bnuM  
The data are the means of three different experiments taking the number of untreated cells 6f) 7*j~  
(vehicle alone) as 100%. wQ?Z y;/S  
ERK and PKB activation were then analyzed by western blot with the corresponding qJ[wVNHh!  
phospho-specific antibodies. To ensure equal amount of protein in each lane, western DWupLJpk;c  
blots against total protein were performed for ERK and PKB. X3HJ3F;==  
The means of optical density (OD) values of the bands detected in western blots of three h>ZU67-   
different experiments are plotted, taking the maximal value of phosphorylation of ERK ^+x,211f  
and PKB as 100%. Au"BDP  
CT3 cells were treated with 25 g ml-1 CsA or vehicle alone for 24 h and then fixed with OmZK~$K_  
paraformaldehyde and stained with Hoescht as indicated in Materials and Methods. Then q@i.4>x  
cells were visualized by phase-contrast microscopy (upper panel) and fluorescence ;i!$rL  
microscopy (lower panel). >=/DCQ$  
The data are the means of three experiments performed on different days taking the O)c3Lm-w  
number of cells at the onset of the experiment as 100%. 8i Ew;I_  
Rats were killed at 4 h and at days 1, 2, 4, 7, 9, 14, 21, and 28 after disease induction Y8for'  
(n=9 each). nPhREn!  
*P<0.05 versus non-nephritic rats. 9Us'Q{CD   
The expression of the CCN3 protein was detected by western blot analysis (n=4 each, PC, 0s6eF+bs  
positive control, M, molecular weight markers). Tsocc5gWZ*  
Original magnifications: 200 in A, E, G, H; 600 in B, C, D, F. :=\`P  
Data are means s.d. of four independent experiments. ?f*>=;7=  
*P<0.05 versus 0.5 h. *~^%s +b  
PDGF-BB and -DD, but not PDGF-AA and -CC, induce a downregulation of CCN3 cFHSMRB|P  
mRNA as determined by real-time RT-PCR and normalized to \~.elKw<U  
glyceraldehyde-3-phosphate dehydrogenase mRNA. Si68_]:^  
Cell number was counted in duplicate using a Malassez hemocytometer }[OEtd{  
Data are means s.d. of four independent experiments. *P<0.05 versus cells treated with S*%:ID|/C2  
MCDB media for 24 h, #P<0.05. =z'533C  
28 caZ EZk#r;  
The curves are adjusted for history of coronary artery disease, CRP, and progression to FYH^axpp  
ESRD. `[~LMV&2U  
Both models are independent of age, gender, baseline GFR and proteinuria, and other A+VzpJ~  
clinical characteristics and traditional and nontraditional cardiovascular risk factors 1~vv<`-  
Correlation between serum bone-specific alkaline phosphatase (bAP) and serum PTH 5#::42oE  
(n=99, P<0.0001, r2=0.190) ?uXY6J"  
Concentration of serum FGF23 concentration before starting and at the end of the 4-h *oAnG:J+M  
hemodialysis procedure in 23 patients, expressed as log10 values (n=23, P<0.0001, >Sm#-4B-  
Student's paired t-test). c3Zwp%  
Immunohistochemistry for the hypoxia marker pimonidazole (PIM) and HIF-2 ; jR{t=da  
arrow=endothelial cell and CD=collecting duct =<U'Jtu6'  
Magnification: 1000. Nrc-@ ]  
A table of .. is developed and significant recommendations are made. D1EHT}  
CONJUGATION !={QL:  
To Indicate Addition ]CgZt' h{  
additionally, again, also, and then, as can be easily understood, besides, equally important, W |+&K0M  
especially, finally, for the same reason, first, further, furthermore, in addition, last, dq3"L!0u  
likewise, moreover, next, second, third, too, evidently, obviously, roughly speaking, "~T06 !F45  
broadly speaking D66!C{  
To Indicate Cause and Effect `(8RK  
accordingly, as a result, consequently, for this reason, hence, in short, otherwise, then, #M92=IH  
therefore, thus, truly 7/BA!V(na  
To indicate Comparison A{Q~@1  
in a like manner, likewise, similarly , alternatively 2_){4+,fu  
To Indicate Concession ,@t#)HV  
after all, although this may be true, at the same time, even though, even so , I admit, 2^&5D,}0  
naturally, of course e{0O "Jd`  
To Indicate Contrast u!cA_,  
and yet, at the same time, but, for all that, however, yet, in fact, in contrast, in the real life, zxvowM  
in spite of, nevertheless, notwithstanding, normally, on the contrary, on the other hand, 6dYUMqQ  
still, traditionally, rather, unfortunately, r'u[>uY  
To Indicate Time Relationships 5<?/M<i  
after a short time, afterwards, as indicated earlier, as long as, as soon as, at last, at length, I^S{V^Ty  
at the moment, at that time, at the same time, before, earlier, currently, immediately, in |*zvaI(}  
the meantime, in recent years, lately, later, meanwhile, often, of late, presently, recently, )X-TJ+d  
soon, shortly, since, thereupon, temporarily, therefore, until, when, while JC4Z^/\.  
29 #:/-8Z(0  
To Indicate Special Features or Examples i- r y5x  
for example, for instance, incidentally, indeed, in fact, in other words, in particular, in j~Ubpf  
practice, specifically, that is, to illustrate, in this respect, theoretically, as mentioned pw1&WP&?3  
before / above -Tr*G4  
To Indicate Summary >]WQ1E[=  
in brief, in conclusion, in short, in summary, on the whole, to conclude -44''w?z  
, in general, to summarize, to sum up, as a result, ultimately, pc<A ,?  
VERB PHASE woR }=\K  
build a .. model 6o ]X.plr  
have been described to FcZ)_m6m  
To assess the functional role of *7 >K"j  
we detected fHwr6"DJ  
build up the key link X7& ^"|:  
Although a number of studies have identified predictors of QOY M/1U  
began a new era in ... <3c|S_|L*m  
can be regarded as / achieved / used to/for / found / obtained through 5R7DD5c[  
can result in w.\#!@kZ!  
carries out ... tasks j#E&u*IR  
production information in order to simultaneously mmrz:_  
contains all information necessary to describe ]AzDkKj  
do not make use of production information /*5lO;!s{  
deals with 9q !./)  
end with failure :0RfA%  
fetch the information from the model directly +cnBEv~y  
has great potential / yet to be resolved / spurred the development of / VCUEzR0  
been recognized as %t\ ~3pw=  
site component / cable of / currently implemented for / demonstrated by an example / ZJ|'$=lR  
finally reached / made equal to / equivalent to / more suitable to / oriented to / ^J Y]w^u  
interpreted as / pointed out / potentially of great benefit in the complex task of / shown (Cjw^P|Y@  
in / used to effectively guide the search 1 ojy_  
makes use of l TRQ/B  
was significantly higher as compared to +_LWN8F  
To assess disease-specific alterations, we measured CA{(x(W\:  
To specifically assess the relationship between c$HZvv  
make up i'bUX=JK  
To determine if D27MT/=7  
This statistically significant difference still Ki:.^  
They positively correlated with {)d{:&*K.  
Present #ig* !  
Our results are consistent with our recent data that suggest that >AT T<U=  
30 bM"crRG"  
contribute to # U j~F  
meets the needs of real life production,/ the current demands placed upon it <>FpvdB  
must be justified H(TY.  
point out c _v;"QZ  
play an important role 3r (i=ac0  
relates to V]$Tbxg  
rely on {b6$F[e   
We performed r g$2)z1  
satisfy the needs ^ |^Q(  
determine the total requirements for the ... 8XU m.nV  
uses ... as a key to search for... Cg! ]x o  
without relying on A#6\5u  
will be available/ performed/ overlooked Z/S7ei@56  
NOUN PHASE FX#fh 2  
a basic technical function of Uxyj\p  
a significant reduction of XPt<k&o1,  
drug variability b&V}&9'[M;  
a critical need 2-<i#nA3  
negatively correlated with /M1 /  
a key / principle feature of dk^Uf84.Gr  
a substantial impact on VkNg Vjg  
an intensive review was conducted [0(+E2/:2  
The analysis of the correlations by M;{btu^a  
an increasing need for expanding the application of .... -$[o:dLO  
an important component / function / aspect / issue / ao|v  
each rule is numbered in sequence B7fV_-p:G  
each of these involves f2JeXsOI  
for this calculation, it is necessary to define d,_Ky#K5b  
in the physical environment / integration of _U"9#<  
in the reality of situations where … U;7Cmti"  
many aspects of {v,O  
most past efforts have been spent on ... E26zw9d  
common sense to a well studied and documented technical field. z|o7k;raH  
sources of additional information on ... are listed 8 pQx6QE  
systematic and rationally structured format ?4kM5NtP  
the basis on which a range of ...operations can be established is shown ;w/|5 ;{A;  
THE basic philosophy / principles of / key element / general hypothesis / candidate list @nuMl5C-`  
of / concept of ... has attracted wide interest / function is concerned with / heart / .@  3  
impact / nature / role / task of / kernel functions 0.9%m7.m  
the number of parts needed to e-Xr^@M*Q  
the above statement means that 3q!hY  
the output data is passed to ^Lx(if WJ  
31 ,[ UqUEO  
the proposed method / underlying principle s7A3CY]->  
the recommendations made in this report, if implemented, should (V:z7  
this information resides in < )dqv0=  
this process is composed of ... different ... operation Md4hd#z  
along with the use of JH4hy 9i  
concerning general aspects of H+ P&} 3  
due to ~]MACG:'  
for later use in generating..., + [JvpDv%  
in turn, AG3iKk??T  
it can be claimed/concluded that e ej:  
it demonstrates the decisions required of Z%Kkh2-uh  
it also provides information to .. # %~PNki  
it becomes essential to * LaL('.>  
let ... be the probability that 1BA/$8G  
once... is written, it is compiled into... 3Kx&+  
suppose it is observed that >1}RiOd3  
this is because sew0n`d1  
this results in a /IO<TF(X  
upon completion of the ... analysis, :i?7RouO  
when the knowledge is of mathematics or quantum physics, it will also be ;p/%)WW  
recorded in books and papers c Y C@@?  
selection of rules for using the tools, for generating operation plans, 6njwrqo  
is another matter of preference, since practice varies greatly. YB`;<+sY  
for the sake of convenience \ah.@s  
correct decision to be reach H%peE9>$  
keeping the number of rules to a minimum. 0r=KY@D  
a good process plan will result exhibiting several characteristics: T3_3k. ,|  
practical solutions U_&v|2o#3  
because of rather small job lot sizes )YtdU(^J$  
Backward reasoning can be used to answer the question "should milling tool be select" j>}<FW-N  
different level of knowledge in the realm of process planning
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