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楼主  发表于: 2009-10-18   

医学SCI 论文经典句子

18 QjF.U8  
Jurkat cells cultured in calcium-free RPMI-1640 medium (Gibco BRL; number URX>(Y}g9^  
22300-107) containing calcium-free 10% FBS were triggered by anti-Fas IgM. The s26:(J [{  
treated cells were harvested at the indicated time points and incubated with Fluo-3-AM at h^?[:XBeav  
a final concentration of 1 micromolar for 30 min at 37°C (Scoltock et al., 2000). The ) dwPD  
labeled cells (50,000 cells per treatment) were then analyzed by exciting the cells at 488 6hAeLlU1  
nm and examining the fluorescence emission of Fluo 3 at 530 nm with a FACS Scan, Wi hQ j  
(Becton Dickinson). A one micromolar concentration of LPA was used as a positive $zy Y"yWRZ  
control for Ca2+ induction. The data thus obtained was analyzed with the software Win s|%mGt &L  
MDI 2.8 and represented as contour plots.The effect of chelating intracellular calcium on Y-gj X$qGo  
translocation of annexin I was studied by culturing Jurkat cells in the presence of 10 CPF d 3 3  
micromolar BAPTA-AM, with or without the addition of anti-Fas IgM. Cells were EX=+TOkAf  
harvested and fractionated as detailed above, and the S-100 fractions were assessed by N[){yaj  
immunoblotting for presence or absence of annexin I. v3Te+oLg  
Mouse bone marrow transduction and transplantation. @X\Sh>H  
Retrovirus-mediated transduction of mouse bone marrow cells was done by published ?dC[VYC\^  
methods49. Prestimulated low-density bone marrow cells were infected with high-titer CZxQ z  
retrovirus supernatant on fibronectin-coated plates. Retrovirus supernatant was generated +\\*Iy'xK  
in the phoenix-gp cells with a mouse stem cell virus–based retroviral vector coexpressing -r/#20Y  
EGFP and HA–RhoH as described50. EGFP+ sorted cells were transplanted by -TjYQ  
intravenous injection into the sublethally irradiated (300 rads with a 137Cs irradiator) $6Nm`[V  
Rag2-/- recipient mice. At 9 weeks after transplantation, thymus, peripheral blood, bone ! |}J{  
marrow, spleen and lymph nodes from each recipient mouse were collected for analysis =}Bq"m  
of EGFP+ chimerism and hematopoietic lineage by flow cytometry. Expression of #HJF==  
HA–RhoH and HA–RhoHF73F83 in EGFP+ sorted thymocytes of recipient mice was f?2Y np=@  
confirmed by immunoblot analysis. D3HE~zkI  
Determination of renal morphology D 7 [n^WtL  
Kidney slices were postfixed in buffered 2% OsO4, dehydrated, and embedded in an /-8v]nRB  
Araldite-EM bed 812 mixture. Large sections were cut perpendicular to the renal capsule, n-}:D<\7  
containing cortex, and medulla. Thin (1 m) sections were analyzed in a blinded manner >XSe  
for morphologic alterations, as previously detailed 3wX{U8mrg  
Patient population ^ B=x-G.  
Patients included in the study met the following criteria: (1) biopsy-proven IMN; (2) A0V"5syY  
creatinine clearance 30 ml per min per 1.73 m2; and (3) persistent proteinuria >5 g per PxfeU2^{0  
24 h despite treatment with an HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) l(v$+  
reductase inhibitor, an ACEi, and/or ARB at maximal tolerated dose for at least 4 months. h$f/NSct2  
The Mayo Institutional Review Board and the Research Ethical Board, University Health uZ]B?Z%y#  
Network, University of Toronto approved the study protocol. All patients gave written ,au64sH  
informed consent. Patients who had been on treatment with prednisone, cyclosporine, or kZU8s'C  
19 79s6U^vv"  
mycophenolic mofetil within the last 4 months or alkylating agents within the last 6 ~XsS00TL`G  
months were not included in the study. Patients with active infection, diabetes, or a [9BlP  
secondary cause of MN (for example, hepatitis B, systemic lupus erythematosus (SLE), cQ6[o"j.  
medications, malignancies) were also excluded. ]1)@.b;QR  
Treatment }sPY+ZjV  
At enrollment, a low-sodium (<4 g day-1) and low-protein (0.8 g per kg per day of >h[tHM O  
high-quality protein) diet was recommended and patients were encouraged to maintain K[~Wj8W0  
the same diet throughout the duration of the study. All patients received a similar nI2}E  
conservative treatment regimen that included loop diuretics to control edema, an l>~:lBO  
HMG-CoA reductase inhibitor, and an ACEi combined with an ARB if tolerated. iL|*g3`-f  
-Blockers and non-dihydropyridine calcium channel blockers, in that order, were added _q6+]  
when required to control systolic blood pressures to <135 mm Hg in >75% of the *uA?}XEfi  
readings. Patients who after a minimum of 4 months of conservative therapy and II>X6  
maximized Ang II blockade had proteinuria >5 g per 24 h received two i.v. infusions of "Y(S G  
rituximab at a dose of 1000 mg on days 1 and 15. To minimize infusion reactions, ;2L=WR%  
patients were premedicated with acetaminophen (1000 mg) and diphenhydramine \RQ5$!O  
hydrochloride (50 mg) orally. In addition, methylprednisolone (100 mg, i.v.) was given / sH*if  
prior to the first rituximab infusion. B-cell depletion was defined as CD19+ count _& KaI }O  
<5 cells per l at any time and B-cell recovery was defined as CD19+ cell count h@{@OAu?  
>15 cells per l. Patients treated with rituximab, who at month 6 had proteinuria >3 g per JYv&It  
24 h and in whom CD19+ B-cell counts had increased to >15 cells per l, received a X@~sIUXx9  
second course of rituximab treatment following the same protocol described above. De>pIN;B>  
Follow-up e% 5!  
In all patients, clinical and laboratory parameters including complete blood counts, &RR Ora  
electrolytes, serum albumin, B-cell flow cytometry for CD19+ B cells, serum ;A- Ef  
immunoglobulin (IgG, IgM, IgA) levels, and a lipid panel were evaluated at study entry *,'"\n  
and at months 1, 3, 6, 9, and 12. Creatinine clearance and protein and creatinine excretion "H G:by  
in the urine were assessed by performing two consecutive 24-h urine collections at each c$e~O-OVD?  
time point. Data were considered accurate when urinary creatinine excretion was 3J(STIxg  
consistent with a complete 24 h collection. The mean of the two measurements was 9 z3Iwl  
considered for the analysis. The presence of HACAs was evaluated at baseline and at (>;~((2  
months 3, 6, 9, and 12. *-P@|eg  
Method / Approach / Study/ Technique G 8NSBaZe  
A discussion is presented of a problem-solving system [fW:%!Y'  
To improve the efficiency of the method, the following approach may be applied. =%]dk=n?TN  
In order to an investigation was made to find the causes of the ]h|GaHiE  
Although large collections of rules and equations have been complied, none are generally L1D%vu`  
accepted D!@c,H  
20 :^ cA\2=  
This approach will be explained and discussed thoroughly in the body of the report. zF{ z_c#3@  
This can be accomplished by -AffKo  
This algorithm to compute the total cost can be described step by step as follows: ^$T>3@rDB  
The above preliminary analysis has provided important information 1$ l3-x  
Various methods have been proposed for selecting an optimum... XS1>ti|<  
These concepts have been applied to +q =/}|  
On the basis of the concept mentioned above, N:d" {k  
This can be achieved by A46y?"]/30  
In addition, tissues were stained for infiltrating lymphocytes (CD20 and CD3), and the Uq[NO JC  
amount of interstitial fibrosis was quantified by histomorphometry. Formalin-fixed, fo 5!d@Nv  
paraffin-embedded sections were cut onto coated glass slides. Following heat-induced ?5 +=  
antigen retrieval, sections were incubated at 20°C overnight with either anti-CD20 9 s>JdAw?  
primary antibody or anti-CD3 primary antibody, both at 1:1000 dilution (Dako, Canada M~wJe@bc  
Inc, Mississauga, Ontario, Canada). After rinsing all sections, pretreatment with 3% 8-po|  
hydrogen peroxide was performed to prevent endogenous peroxidase activation. Sections hDfsqSK0 /  
were incubated with a secondary rabbit anti-mouse antibody linked with avidin–biotin { \Q'eL8  
complex. Sections were counterstained with hematoxylin and examined by light _STN^   
microscopy. y=q\1~]Z  
The HACA assay is a proprietary bridging enzyme-linked immunosorbent assay nC2A&n&>  
performed at Genentech Inc. that measures the antibody response to rituximab in human F7L+bv   
serum samples. 8wwD\1pLS  
In all patients, clinical and laboratory parameters including complete blood counts, jYuH zf  
electrolytes, serum albumin, B-cell flow cytometry for CD19+ B cells, serum ^TnBtIU-B  
immunoglobulin (IgG, IgM, IgA) levels, and a lipid panel were evaluated at study entry q(_pk&/  
and at months 1, 3, 6, 9, and 12. 3C2L _ K3  
This fact suggests that a new concept NCd_h<}|6F  
This was accomplished by taking ... Ean@GDLz8  
The preparatory stage is very time consuming process. - x@mS2  
Test are performed for validity, completeness, and compatibility jsc1B  
There is little hope of achieving successful ... $^}?98m  
There has been an increasing awareness of the potential of using most ..so far made have quVTqhg"  
not taken this approach, with the exception of 27G6C`}  
Only a few studies can be found. ^6oz3+  
It is a very tedious process to go through XeKIue@_  
It is only when .. has been completed that .. may be effected %fo+Y+t  
The entire interpretation process is conducted in one's head. 5fA<I _ D  
These approaches are sometimes very tedious. W{Ine> a'  
Several techniques can be used O2-9Oo@#,  
A polynomial parametric model can be written as [the following]/[follows]: bBn4m:  
A xx model is constructed/formulated using xx. $&I##od  
A xx model represents an xx by its xx. d b'Jl^  
A process decision model captures the logic essential to /Z94<}C6b  
From the equation above, xx is equal to the summation of xx times the ... [?Wt ZM^q  
21 )xy1 DA  
The validity of a xx model can be checked using Euler's formula. 67wY_\m9I  
Given a model, one can mathematically determine whether ... or ... MuQyHEDF  
Equations for xx need to be derived and implemented in the system. @7e h/|Y,  
A number of heuristic rules have been developed for :GBWQXb G  
Optimum .. techniques can be made more reliable by ... so that 4p\<b8(9>  
An algorithm based on the characteristic ... is used to determine ) wtVFG  
Euler's formula states the following: (sHqzWh  
The completed model should agree with the formula. i<@6f'Kir  
For manufacturing purposes, a detailed and precise model of the object is necessary Ri=>evx  
Engineering design models are very well defined; therefore, 4 |9M8ocR  
To keep the domain narrow enough to be implementable, yet wide enough to be useful. .&:y+Oww~  
Point of View `z=U-v'H)D  
from an implementation standpoint, EnnT)qos  
From the point of view of this application, l=,\ h&  
From this point of view, Zadeh suggested an inference rule named xxx (CRI for short). Y&!M#7/'J3  
Information is the meaningful interpretation and correlation of some aggregation of data %{ WZ  
in order to allow one to make decisions. ~47Bbom  
From a practical point of view, the computational aspects of an FLC require a ,#:*dl  
simplification of the fuzzy control algorithm. PLD6Ug  
The use of a hammer to insert screws, although partly effective, tends to distort, destroy, D;VQoO  
and generally defeat the purpose of using a screw [Kusiak AI Implications for CIM ^PA >t$  
p.129] g S xK9P  
Statistical analysis ~'>RK  
Data were analyzed by one-way analysis of variance comparing the three conditions {C3AxK0  
(sham operation, ischemic AKI, and bilateral nephrectomy) at each time point. If tl9=u-D13@  
significant F-statistic from analysis of variance existed, this test was followed by Dunnett \9k$pC+l  
post hoc multiple comparison procedure with sham operation as the control group. For all x}o]R  
other comparisons, Student's t-test was used. A P-value of <0.05 was considered as jm9J-%?  
statistically significant. _%^t[4)q  
Values are expressed as means s.e.m. and significance was evaluated by Mann–Whitney {];4  
U test using GraphPad Prism, version 4.0 software (GraphPad Software Inc., San Diego, 7LMad %  
CA, USA). B~z P!^m  
All values are expressed as means s.d. Statistical significance (defined as P<0.05) was %~%1Is`4J  
evaluated using analysis of variance and Bonferroni t-tests, and the two-tailed Pearson's ~um+r],@@  
test, where appropriate. [)UF@Sq4+Q  
Data are expressed as mean s.d., median and interquartile range, or frequencies, as 9Br2}!Ny  
appropriate. Variables who deviated from the normal distribution (positively skewed) DC S$d1  
were log-transformed (log10) before the correlation study. lOp/kGmn+  
Data are represented as means s.e.m. Student's t-test and multiple comparisons with t-test pl62mp!  
post hoc analysis of variance were used as indicated below, for the comparison of SR>Sq2cW0  
22 e  iS~*@  
morphological, immunohistological and functional parameters. Statistical significance  |F5^mpU  
was set at P<0.05. AMqu}G  
The primary efficacy parameter was defined as change in urinary protein excretion from i(.c<e{v~  
baseline (week 0) to 12 months after treatment. The 12-month changes were tested tm\ <w H  
against zero using the paired t-test. Secondary end points included 6-month changes in N{n}]Js1D-  
protein; the number with PR or CR at 6 or 12 months; and changes in glomerular 4f,D3e%T|  
filtration rate (GFR), serum albumin, and lipid profiles. Study sample size was based on pJ7wd~wF*  
the desire to have 80% power to detect a drop in urinary protein of at least 2.0 g day-1. Bu?"b=B*  
Assuming a two-sided hypothesis test performed at a significance level of 0.05 and an s.d. wqo2iRql  
of urinary protein change of 2.5 g, it was determined that 15 patients were required.2 @(.?e<  
Definition of remission status is according to the criteria established by Cattran et al.30 ]7kq@o/7  
CR was defined as proteinuria <0.3 g per 24 h, PR as proteinuria 3 g per 24 h, and a ixN>KwH  
>50% reduction in peak proteinuria and non-response as <50% reduction in peak cqeId&Cg  
proteinuria. Any patient reaching a CR or PR was considered a treatment success S |T:rc(~  
The statistical significance of differences for the mean values of cytokine concentration EVmQ"PKL'  
and T cell proliferation was determined with Student's t-test. Differences with a P value s<0yQ-=.?N  
of less than 0.05 were considered significant. L]0+ u\(  
Statistical significance was determined by Student's t-test. Data with a P value of less xMg&>}5  
than 0.05 were considered significant. OB3AZH$  
In vitro and in vivo experiments were analyzed by the two-tailed Student's t-test, with P "Gcr1$xG8!  
values less than 0.05 considered statistically significant. k}T~N.0  
The significance of differences among groups was determined by Student's t-test and mz\ m^g3  
one-way analysis of variance (SigmaStat software; Jandel). 82/iVm1  
Comparisons #UWQ (+F  
As well, the pros and cons of these representations from a process planning point of view Jb tbW &EH  
will be discussed. {Z?!*Ow  
The method of using xx to implement xx described by Zadeh (1973) appeared more 0]HK (,/h  
suitable }+=@Ci  
As discussed [in the previous section]/[preciously], _v $mGZpGY  
Relation 'M'LJ.,"/  
We can not invert F' directly because it defines a many-to-one mapping. "V:UQ<a\  
The relationships appear very complicate q?0goL  
Lifting tasks involve complex and imprecise relationship between the task variables and iTK1I0  
the human operator's characteristics. 6)bfd^JYn  
These methods are based on the relationship between ... and ... Tf[ ]vqa`G  
The fundamental concept of a fuzzy rating language is that we can establish a relationship 5rcno.~QO  
among terms such as high, medium, and low, and then modify these relationships. rvfl~<G*  
This article will thus mention the latter as well as the former. d*AV(g#B  
The former two bear a close relation to a fuzzy Cartesian product. TX +t   
Importance b QeYFY#^  
D@ lJ^ +  
23 LpiLk| 2i  
The emphasis is on an implementation of a general approach to rule based decision SwL\=nq+~  
making. ujSzm=_P  
Consideration / Attention TDs eWdA  
Careful evaluation is necessary to ensure @]7\.>)  
Such a formulation does not change further considerations. YS~x-5OE\  
Considerable attention has been paid to g[n8N{s  
Attention should be paid to an important finding of this investigation. Y\=:j7'  
Caution should be exercised in this process to avoid ... 9c6V&b  
Primary consideration is given to ... components, though others can be accommodated GhT7:_r~  
After ... has been defined by ..., a carefully analysis is carried out/performed to determine G@'0vYb#  
A number of factors such as ...need to be taken into consideration before making the -I*A  `M  
appropriate decision. j AE0$u~.  
It should be noted that -jy"?]ve.  
It is important to point out that ... u0e#iX  
These considerations have heightened interest in the possibility of providing ... }X?*o `sW  
We should stress the fundamental importance of the xx :6 fQE#(s&  
Results. +g[B &A!d+  
Advantages / Disadvantage .R'i=D`Pz  
One of the major advantages of this new measure of xx is that it can be applied to the -T8 gV1*(<  
experimental study of ?X1#b2s  
One advantage of using a .. is the ease of preparing it. rZv+K/6*M  
It has a very fast decision making process ;cr6Xop#?  
All the algorithms involve mostly logical operations. |DD?3#G01  
It can be easily and without additional cost implemented in a microprocessor;based lo:~~l  
environment. Z]f2&  
It can reduce the waste of designing from scratch. mmL~`i/  
The advantages of using a xx to represent xx are the following: 0 l@P]_qq`  
However, xx is not without its shortcomings. 6hK" k  
In most cases, the xxx shows an improvement over the existing xxx. >G2 -kL_  
Compared to the existing xx, the impacts of the xx are generally reduced by 5% to 9%. PNgMLQI 6  
The "best case" results shows a savings of 6% to 9%. c5e\ckqm^  
Most of the existing works based on xx approach can only recognize a xx . 6 W;k IoB  
Most of the above methods are computational expansive and limited to xx. bOMP8{H,  
Some other advantages of xx are the following: R+0"B  
The problem is the limitation of this method to a limited domain of parts. .~L4#V{c~  
It proved limited in application because it demanded precision in system modeling that v :MS0]  
was impossible in practice. PV6 *-[  
There are advantages to be gained in the structuring of costs and benefits, the use of xx, \)ZX4rs{8  
The disadvantages of this method are also disadvantages of conventional xx approaches. Y&f[2+?2NK  
This combines the best features of both techniques $L8s/1up  
24 x[W]?`W3r~  
Hopefully, this tool can be as the reference framework of for developing a xx platform, h_G|.7!  
and helping the administration, marketing, and knowledge management activities in X]MM7hMuR  
virtual communities. +1jqCW  
Results /{ MH'  
An improvement on the result shown above can be made by based on the data provided @-@rG>y^:  
Recent work has identified yn ":!4U1  
Time-dependent changes of (Zn3-t*  
Cyclosporine-induced cell death is triggered by a non-classical phosphoinositide 3-kinase mRnzP[7-\)  
and does not require ERK activation. t>GLZzO  
Much of the current work on YZ:'8<  
Discussion of these theories is beyond the scope of this review 5`'au61/2  
Based on the information contained in this h8= MVh(I  
The result can be categorized into nine classes YSz$` 7i  
The results are illustrated by an example _E<O+leWf  
The experimental results for each xx time are reported in Table 2. ]#-/i2-K  
From the results obtained so far, it seem that [xs)u3b  
Because of the inaccuracy of the ..., a conclusion cannot be drawn as Ysc|kxLb  
Although much effort has been made to., this reality is far from completion. awSS..g}L  
The results indicate that the total benefits are higher than the total costs. Hs` #{W{.  
Their results may then serve as guidelines for lower level models, less fuzzy and more zmfRZ!Eh  
detailed. vH?+JN"A  
Conclusion g*r{!:,t  
From the discussion, one may conclude that ... G5nj,$F+  
The first indication that iI@Gyq=  
In summary, we have shown that Zc\h15+P  
These results suggested that Jyz$&jqyr'  
Our findings have shown that vLK\X$4  
Our observations have provided HSr"M.k5  
This study reveals following five main findings: *CPB5s  
To our knowledge, this is the first immunohistochemical report of both PIM and HIFs in Yr"!&\[oz  
the diabetic kidney. 9p\wTzA  
As mentioned above, Q!2iOvK  
Moreover, we demonstrate that, K@j^gF/0B  
A number of studies have elaborated a body of knowledge about :U-US|)(2  
There are findings to indicate that z5yb$-j  
On a descriptive level, there is agreement that for kO,VayjT  
One issue that became particularly evident from our study is 9?l?G GmQ  
Our data are in agreement with the findings and models presented by previous k}f<'g<H  
publications, in particular those of Jones et al Lr24bv\  
According to our observations, ]lB3qEn<  
…was not evident from our data A}#] g>L  
25 CNpe8M=/3  
It could therefore be concluded that 1 ~B<  
our data illustrate that X8,7_D$  
All these different aspects, as listed above, lead to ,3J`ftCV  
In fact, it has already been speculated that P"V{y|2  
It should be furthermore mentioned in this context that 94p:|5@  
To identify and verify the essential factors triggering developmental renin expression, it z*dQIC  
is of interest now to study the development of intrarenal renin expression in mice with n2jvXL Jq  
defined gene mutations, using the results of this study as a reference system. :Er^"9'A2  
In conclusion, using several approaches, we demonstrated that pT3X/ ra  
But we have not provided formal proof yet that ^8';8+$  
In summary, our results suggest that jIx8k8  
In conclusion, we identify DD/>{kff  
A better understanding of such regulatory systems may yield novel therapeutic m?D <{BQ;  
approaches to glomerular diseases. .)"_Q/q  
Preliminary results of short-term studies (2 weeks) indicate that Q )LXL.0h  
However, we believe that ?Y? gzD  
However, despite our extensive measurements of these parameters, we found no #gW /qJ  
relationship between response and initial proteinuria, time to B-cell recovery, the V>b2b5QAH,  
development of antibodies to rituximab, B-cell count in the kidney tissue, nor the degree _pz,okO[V  
of tubular interstitial damage present. A clear conclusion for efficacy, however, cannot be ,:#,}w_HyO  
obtained from an uncontrolled study, and definitive claims of efficacy should be reserved lZ|L2Yg3uB  
for rigorous, prospective, controlled, and randomized trials with the use of rituximab and z-EwXE  
that will also look for factors that may determine its efficacy in IMN. YSR mt/   
It has become increasingly clear that q"gqO%Wb|  
Form the above discussion, the conclusion can be reached that _j:UGMTi(U  
The conclusions drawn are also valid V]I:2k5  
In conclusion to this, it becomes obvious that the problem of xx lies not only in... /y}"M  
We have attempted to introduce some concepts associated with a theory of L'zE<3O'3  
Considerable more work, hopefully, will be done in this area j$3rJA%rN  
Employing the compositional rule of inference, the assessment of the xx compatibility in ifXW  
achieving prescribed xx projectiles in any level of the hierarchy is made possible. di~]HUZh)  
This paper has presented a theoretical and experimental study of the xx process and xx I Byf_E;r  
concept. Fi mN?s  
The experimental research results will hopefully serve as useful feedback information for "(s6aqO$  
improvements for xx work. 6iG(C.b  
The scope of this contribution was to introduce a xx method.  n;wwMMBM  
This has a tremendous impact on our understanding of C:]s;0$3'9  
Significant progress has been made in advancing RNAi therapeutics in a remarkably 4&Byl85q  
short period of time qAoAUD m  
To date, little is known about… 9A~>`.y  
Irrespective of the mechanisms and the molecules –which are still largely unknown – =I2@/,  
involved in MSC functions, current data suggest that ?w-1:NW jt  
To better understand 3y99O $EAc  
26 pz{'1\_+9  
Future Research (常用于conclustion 的结尾) +R!zs  
Thus, first extension of the approach could be, cJCU*(7&  
Further studies are needed to determine the importance of ( #* "c  
Some improvements to the scheduling aspect of the model may be brought through i}v9ut]B  
additional levels in the hierarchy for more detailed representation of the scheduling SxJ$b  
activity. iv*V#J>  
It also unveiled new aspects of the role played by thyroid hormone in response to injury ow$q7uf  
and tissue repair. K UKACUL  
In the near future, the ongoing clinical trials with siRNAs for macular degeneration and d+;gw*_Ei  
RSV may reveal the exciting potential of RNAi therapeutics as the next major class of LXj5R99S  
drug molecules. %%%S"$t  
In the next few years it should become clear whether jfD1  
It should be an exciting time for researchers seeking to harness this powerful endogenous /` [!_4i  
pathway to treat human disease. F30 ]  
Acknowledgement lT'9u,6   
The authors are grateful to the insightful work provided by Mr. John W. Harney and all y500Xs[c  
of the fellows and students that contributed with some of the studies discussed in this Dw_D+7>(v  
article (CJ.BHu]  
I would like to express my gratitude to all those who gave me the possibility to complete im]g(#GnKh  
this thesis. x({H{'9?  
I want to thank the Department of Transport of the former Interim Government of wJeG(h  
Namibia for giving me permission to commence this thesis in the first instance, to do the h1[WhBL-O  
necessary research work and to use departmental data AVl~{k|  
This work was supported by NIH grants AI058695 and AI056900. We thank members of ,+`HQdq  
the laboratory and our collaborators for many useful suggestions. "5Uh< X  
We thank J. Maraganore and B. Greene for critical reading of the manuscript, M. g5Z#xszj+  
Duckman for graphics assistance and A. Capobianco for word processing assistance. C [uOReo  
Author contributions 8X$LC  
All authors were involved in experimental planning and data analysis and contributed to +Qxu$#  
manuscript preparation. l|/LQ/  
Tables and Figures K_/zuTy  
Figure 7-1 sketches these relationships. (`BSVxJH  
The graphical representation of these functions is shown in Figure 1. )$ Mmn  
The xx may be depicted as in Figure 1. L';MP^  
Figure x shows the schematic diagram of the X2|Y  
Figure 1 though 2 provide a ... that SqA J-_~  
the architecture of this expert system for .... is illustrated in Figure 2. E Y<8B3y  
Figure 2 gives the outline of an ... system zSEs?  
Table shows the H)w(q^i  
27 N2'qpxOLI  
Time-dependent changes of #H?t!DU  
as shown in Table 1 and 2 WE6a'  
This concept is illustrated in Figure 2 "9EE1];NT  
At the top of Table xx are shown two blocks of data. mW2,1}Jv  
Each table or matrix has constructs xx through xx as row;headings, OLGBt  
xx through xx as column;headings. |BT MJ:B  
Time course of calculated renin immunoreactive tissue volumes during development of F~:O.$f]G  
the mouse kidney. Data are single values per time point. They were derived from the '^BV_QQ  
kidneys shown in Figure 1. le+R16Z  
Yellow color indicates 2~[@_  
PCT3 cells were pretreated with 50 M PD98059 or 10 M U0126 for 30 min and then -oeL{9;  
cells were stimulated with 25 g ml-1 CsA for 6 h. ERK and PKB activation were E!BPE>  
analyzed by western blot using phospho-specific antibodies. NjPDX>R\K  
ERK and PKB activation were measured by western blot using phospho-specific ]<(]u#g_d  
antibodies bNFLO Q  
The data are the means of three different experiments taking the number of untreated cells UJ-?k &j,  
(vehicle alone) as 100%. >Vy>O &r  
ERK and PKB activation were then analyzed by western blot with the corresponding b|Q)[y]  
phospho-specific antibodies. To ensure equal amount of protein in each lane, western du$|lxC  
blots against total protein were performed for ERK and PKB. J q{ 7R  
The means of optical density (OD) values of the bands detected in western blots of three M8H5K  
different experiments are plotted, taking the maximal value of phosphorylation of ERK '(? uPr  
and PKB as 100%. '$4O!YI9@  
CT3 cells were treated with 25 g ml-1 CsA or vehicle alone for 24 h and then fixed with Kvsh  
paraformaldehyde and stained with Hoescht as indicated in Materials and Methods. Then o6f_l^+H  
cells were visualized by phase-contrast microscopy (upper panel) and fluorescence =@TQ>Qw%b  
microscopy (lower panel). ^pM+A6 XY  
The data are the means of three experiments performed on different days taking the 7t,t`  
number of cells at the onset of the experiment as 100%. "V{yi!D{<  
Rats were killed at 4 h and at days 1, 2, 4, 7, 9, 14, 21, and 28 after disease induction ~|wbP6</:-  
(n=9 each). 0$7.g!h?  
*P<0.05 versus non-nephritic rats.  L4uFNM]  
The expression of the CCN3 protein was detected by western blot analysis (n=4 each, PC, iC iZJ"  
positive control, M, molecular weight markers). - 0?^#G}3}  
Original magnifications: 200 in A, E, G, H; 600 in B, C, D, F. cb5,P~/q  
Data are means s.d. of four independent experiments. :eo  
*P<0.05 versus 0.5 h. 8kP 3+  
PDGF-BB and -DD, but not PDGF-AA and -CC, induce a downregulation of CCN3 N'xSG`,Mg  
mRNA as determined by real-time RT-PCR and normalized to `9k0Gd  
glyceraldehyde-3-phosphate dehydrogenase mRNA. KR7@[  
Cell number was counted in duplicate using a Malassez hemocytometer fib}b? vk  
Data are means s.d. of four independent experiments. *P<0.05 versus cells treated with ko5V9Drc  
MCDB media for 24 h, #P<0.05. Wu)>U  
28 u^029sH6j  
The curves are adjusted for history of coronary artery disease, CRP, and progression to l7De6A"  
ESRD. TIvRhbu  
Both models are independent of age, gender, baseline GFR and proteinuria, and other 'U'yC2BI n  
clinical characteristics and traditional and nontraditional cardiovascular risk factors :kfp_o+J  
Correlation between serum bone-specific alkaline phosphatase (bAP) and serum PTH (rg;IXAq%  
(n=99, P<0.0001, r2=0.190) sUE?v9  
Concentration of serum FGF23 concentration before starting and at the end of the 4-h =~p>`nV  
hemodialysis procedure in 23 patients, expressed as log10 values (n=23, P<0.0001, XV^1tX>f{  
Student's paired t-test). XkyKBg-  
Immunohistochemistry for the hypoxia marker pimonidazole (PIM) and HIF-2 ; "n*~Mj Ny  
arrow=endothelial cell and CD=collecting duct Ao/ jt<  
Magnification: 1000. r [ K5w  
A table of .. is developed and significant recommendations are made. N<DGw?Rl  
CONJUGATION jMpD+Mb  
To Indicate Addition WVLHfkN  
additionally, again, also, and then, as can be easily understood, besides, equally important, VcoOeAKL  
especially, finally, for the same reason, first, further, furthermore, in addition, last, )Y)pmjZaG  
likewise, moreover, next, second, third, too, evidently, obviously, roughly speaking, >a }f{\Q  
broadly speaking Y/7 $1k  
To Indicate Cause and Effect ZI=v.wa  
accordingly, as a result, consequently, for this reason, hence, in short, otherwise, then, !>1@HH?I\/  
therefore, thus, truly 9*a=iL*Nw  
To indicate Comparison -{ZWo:,r~q  
in a like manner, likewise, similarly , alternatively ` Jdb;  
To Indicate Concession ,{c?ymw?  
after all, although this may be true, at the same time, even though, even so , I admit, 71\xCSI1w&  
naturally, of course YIjTL!bA"  
To Indicate Contrast Z4\=*ic@  
and yet, at the same time, but, for all that, however, yet, in fact, in contrast, in the real life, |iwP:C^\mJ  
in spite of, nevertheless, notwithstanding, normally, on the contrary, on the other hand, S9#)A->  
still, traditionally, rather, unfortunately, >FkWH7  
To Indicate Time Relationships 0L/n?bf  
after a short time, afterwards, as indicated earlier, as long as, as soon as, at last, at length, Q@HopiC  
at the moment, at that time, at the same time, before, earlier, currently, immediately, in ^ (FdXGs[  
the meantime, in recent years, lately, later, meanwhile, often, of late, presently, recently, yNbjoFM.i  
soon, shortly, since, thereupon, temporarily, therefore, until, when, while T~='5iy|  
29 1aTB%F  
To Indicate Special Features or Examples 1{JV}O  
for example, for instance, incidentally, indeed, in fact, in other words, in particular, in r% +V8o  
practice, specifically, that is, to illustrate, in this respect, theoretically, as mentioned t )}scf&^x  
before / above [;J>bi;3N  
To Indicate Summary lx7Q.su'  
in brief, in conclusion, in short, in summary, on the whole, to conclude Qr{E[6  
, in general, to summarize, to sum up, as a result, ultimately, :w^Ed%>y7  
VERB PHASE 73\JwOn~  
build a .. model {fjdr  
have been described to "9Q40w\  
To assess the functional role of mrF58Uq;A  
we detected aq@ 8"b(.  
build up the key link yL-YzF2  
Although a number of studies have identified predictors of 2HsLc*9{4  
began a new era in ... @X;!92i  
can be regarded as / achieved / used to/for / found / obtained through Tq[kl'_  
can result in '|8} z4/g  
carries out ... tasks T~##,qQ  
production information in order to simultaneously U!*M*s  
contains all information necessary to describe EWkLXU6t  
do not make use of production information 9\BT0kx  
deals with a C\MJ 9  
end with failure Gsq00j &<Z  
fetch the information from the model directly >\P@^ h]  
has great potential / yet to be resolved / spurred the development of / FE (ev 9@  
been recognized as fY51:0{  
site component / cable of / currently implemented for / demonstrated by an example / yy i#Mo ,  
finally reached / made equal to / equivalent to / more suitable to / oriented to / v50bdj9}k  
interpreted as / pointed out / potentially of great benefit in the complex task of / shown Vr`R>S,-  
in / used to effectively guide the search WrxP  
makes use of AQ,' 6F9  
was significantly higher as compared to p93r'&Q  
To assess disease-specific alterations, we measured 7XTkX"zKj  
To specifically assess the relationship between z<U-#k7nz  
make up +eK"-u~K  
To determine if -pf}  
This statistically significant difference still +./H6!  
They positively correlated with .f%fHj  
Present bMg(B-uF7  
Our results are consistent with our recent data that suggest that c'>/   
30 AN8`7F1  
contribute to 4o <Uy  
meets the needs of real life production,/ the current demands placed upon it I7|Pi[e  
must be justified rzhWw-GY  
point out %p ^wZtm  
play an important role ;4R$g5-4X  
relates to +7D|4  
rely on LSa,1{  
We performed #^l L5=  
satisfy the needs Zd^rNHhA  
determine the total requirements for the ... y I HXg#  
uses ... as a key to search for... #I{h\x><?  
without relying on .b3Qfxc>  
will be available/ performed/ overlooked eJF5n#  
NOUN PHASE XX7{-Y y  
a basic technical function of CJ/X}hi,  
a significant reduction of [pC$+NX  
drug variability ypbe!Y<i]  
a critical need _GXk0Ia3`  
negatively correlated with [.O?Z=5a[V  
a key / principle feature of 4,bv)Im+ `  
a substantial impact on bZnuNYty75  
an intensive review was conducted S@jQX  
The analysis of the correlations by I?h)OvWd  
an increasing need for expanding the application of .... Vi>,kF.f V  
an important component / function / aspect / issue W3]_m8,Z  
each rule is numbered in sequence 6 bomh2  
each of these involves Ee)T1~;W  
for this calculation, it is necessary to define 8UW^"4  
in the physical environment / integration of HKU~UTRnZ  
in the reality of situations where … ~k\fhx  
many aspects of j5K]CTz#  
most past efforts have been spent on ... txJr;  
common sense to a well studied and documented technical field. |*oZ _gI  
sources of additional information on ... are listed I3=%h  
systematic and rationally structured format !Z`j2 e}  
the basis on which a range of ...operations can be established is shown "8c@sHk(w  
THE basic philosophy / principles of / key element / general hypothesis / candidate list T?:Rdo!:u  
of / concept of ... has attracted wide interest / function is concerned with / heart / ~?D4[D|sB  
impact / nature / role / task of / kernel functions yL%K4$z  
the number of parts needed to )` SE S."  
the above statement means that `{eyvW[Ks  
the output data is passed to u\E?Y[1  
31 Gn\_+Pj$  
the proposed method / underlying principle N 96jJk  
the recommendations made in this report, if implemented, should ~J5+i9T. )  
this information resides in 03xa'Of>  
this process is composed of ... different ... operation Ot:CPm@  
along with the use of ~3UQ|j  
concerning general aspects of b^ L \>3  
due to 3b*cU}go  
for later use in generating..., z3`-plE  
in turn, 7q?9Tj3  
it can be claimed/concluded that p%iGc<vHX  
it demonstrates the decisions required of JCz@s~f\y  
it also provides information to .. xgABpikC^  
it becomes essential to 8 ![|F:  
let ... be the probability that 2uG0/7  
once... is written, it is compiled into... ]NjX?XdX<  
suppose it is observed that t]>Lh>G  
this is because (]b!{kS  
this results in a 7Ok;Lt!x  
upon completion of the ... analysis, 3l)hyVf&  
when the knowledge is of mathematics or quantum physics, it will also be P6!jRC"52'  
recorded in books and papers Lk~ho?^`  
selection of rules for using the tools, for generating operation plans, Fkvl%n  
is another matter of preference, since practice varies greatly. &F\?  
for the sake of convenience }tsYJlh5  
correct decision to be reach _Xnqb+  
keeping the number of rules to a minimum. :80Z6F.k`  
a good process plan will result exhibiting several characteristics: [E+$?a=  
practical solutions ul u9'ch  
because of rather small job lot sizes x;-. ZVF  
Backward reasoning can be used to answer the question "should milling tool be select" hy}8Aji&  
different level of knowledge in the realm of process planning
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